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Mode of Action (MoA)
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None
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Evidence
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A high-throughput reverse genetics approach (Toxin Inhibition by Conjugation, TIC) was used to identify RcaT inhibitor. The TIC (Toxin Inhibition by Conjugation) platform is a high-throughput genetic screen designed to identify blockers of toxin activity. It involves conjugating an E. coli overexpression library into a strain expressing the toxin gene (rcaT) under an inducible promoter. Strains that regain growth upon co-induction of the toxin and a library gene are considered to carry candidate RcaT inhibitors.
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MoA Category
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unknown
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Salmonella enterica type II Retron (Sen2)
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Relevant publication(s)
DOI
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10.1038/s41586-022-05091-4
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Metagenome
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Defence system(s) inhibited by the protein
Defences
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Retron
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