|
Mode of Action (MoA)
|
stabilises the antitoxin MazE by affecting the ClpP proteases responsible for its degradation.
|
|
Evidence
|
In P1 phd‑doc tests, cells expressing wild-type rexB survived plasmid loss at high temperature (ECO_0000017), whereas rexB mutants (nonsense or deletion) did not. Induction of ppGpp—or amino acid starvation via serine hydroxamate—to activate mazEF leads to ~90% killing; but rexB, either from plasmid or a λ lysogen, restores survival up to ~60–100% depending on the setup. Pulse-chase experiments demonstrated that λRexB extends the half-life of the antitoxins Phd (from P1) and MazE (from E. coli), both substrates of ClpP proteases (ClpPX and ClpPA, respectively)
|
|
MoA Category
|
uncategorised
|
|
Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
|
Escherichia coli type II TA system MazEF
|
|
Relevant publication(s)
DOI
|
10.1073/pnas.95.26.15481
|
|
Other components of the anti-defence system
Multicomponent System
|
-
|
|
Known structure in PDB
PDB ID
|
-
|
|
Genome(s) encoding the protein
Protein Source
|
Enterobacteria phage lambda
|
|
Defence system(s) inhibited by the protein
Defences
|
TA
|