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Mode of Action (MoA)
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Triggers Gasdermin defence
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Evidence
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The experimental evidence supporting RIIB activity includes: (1) RIIB translation mutants with reduced protein levels showed graded susceptibility to rex-mediated restriction, correlating with the amount of RIIB produced. For example: (a) rII mutants expressing 46% and 39% of wild-type RIIB had partial restriction. (b) Mutants expressing 7%, 3%, or 0% (deletion mutant r638, ECO_0001038) were fully restricted. (2) The restriction level correlated quantitatively with RIIB levels and rex expression levels (modulated via IPTG induction or promoter strength). (3) Complementation data showed that wild-type RIIB activity is required to overcome rex restriction, and that loss of RIIB leads to arrest of phage development in rex⁺ hosts. (4) Temperature-dependent effects further confirmed RIIB’s role: restriction by rex was stronger at 42°C and weaker at 30°C, matching expected temperature sensitivity of translation or protein stability.
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MoA Category
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unknown
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli RexAB
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Relevant publication(s)
DOI
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10.1128/jvi.61.12.3790-3794.1987
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Enterobacteria phage T4
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Defence system(s) inhibited by the protein
Defences
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RexAB
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