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Mode of Action (MoA)
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cleaves the signalling molecule SAM-AMP used by type III CRISPR systems
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Evidence
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DAN18478 was identified as a candidate phage-encoded SAM-AMP lyase from a human microbiome viral metagenome. It was recombinantly expressed in E. coli and purified to near-homogeneity. The purified protein exhibited SAM-AMP lyase activity, producing MTA-AMP. The phage enzyme functionally substituted for C. botulinum SAM-AMP lyase in a plasmid interference assay involving the B. fragilis Cmr/CorA type III CRISPR system, supporting its functional role in neutralizing CRISPR immunity.
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MoA Category
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degrades or sequesters molecules utilised by host defence systems
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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None
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Relevant publication(s)
DOI
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10.1093/nar/gkaf655
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Metagenome
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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