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Mode of Action (MoA)
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binds the signalling molecule cGAMP.
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Evidence
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Deletion of vs.4 (ECO_0001038) in the T4 phage reduced its ability to infect bacteria lacking functional Cap2, indicating that Vs.4 helps evade CBASS immunity. Complementation with wild-type vs.4 restored phage infectivity, supporting its role as a CBASS antagonist. Isothermal titration calorimetry (ECO_0001825) revealed that Vs.4 binds cGAMP with high affinity (Kd ≈ 30 nM). Crystal structure (ECO_0001034) showed that Vs.4 forms a hexamer, each binding three cGAMP molecules. Mutations in Vs.4 that disrupt cGAMP binding or hexamerization (e.g., Y8A, F82A, A77I) abolishes CapV inhibition (ECO_0000015).
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MoA Category
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degrades or sequesters molecules utilised by host defence systems
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli type II CBASS
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Relevant publication(s)
DOI
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10.1038/s41586-023-05862-7
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Other components of the anti-defence system
Multicomponent System
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-
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Known structure in PDB
PDB ID
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7UQ2_A
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Genome(s) encoding the protein
Protein Source
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Enterobacteria phage T4
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Defence system(s) inhibited by the protein
Defences
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CBASS
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