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Mode of Action (MoA)
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prophage encoded anti-defence protein counteracting self-encoded defence system, TerI. TerI targets the terminase complex of invading phages, and anti-TerI1 and anti-Terl2 counteract Terl through direct interaction during prophage induction to allow virion production.
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Evidence
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Anti-TerI1 (encoded by LMRG_01518) was identified as a self-immunity factor that neutralizes the anti-phage defense protein TerI. Its function was revealed through a suppressor screen using terI-expressing bacteria repeatedly infected with phage ϕ10403S, which selected for a phage mutant with enhanced early gene expression and resistance to TerI inhibition. Overexpression of LMRG_01518 in terI-expressing cells restored virion production to wild-type levels, confirming its counter-defensive role. Deletion of LMRG_01518 (ECO_0001038) impaired virion production upon prophage induction, resulting in capsids devoid of DNA, a phenotype rescued by inactivating terI expression. This demonstrates that Anti-TerI1 counteracts TerI activity in vivo. When overexpressed alone, Anti-TerI1 also rescued virion production during exogenous phage infection, confirming its ability to neutralize TerI's anti-phage activity.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Prophage ϕ10403S TerI defence system (active in Listeria monocytogenes)
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Relevant publication(s)
DOI
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10.1101/2025.02.27.640495
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Other components of the anti-defence system
Multicomponent System
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anti_rerI2
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Prophage ϕ10403S
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Defence system(s) inhibited by the protein
Defences
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TerI
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