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Mode of Action (MoA)
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prophage encoded anti-defence protein counteracting self-encoded defence system, TerI. TerI targets the terminase complex of invading phages, and anti-TerI1 and anti-Terl2 counteract Terl through direct interaction during prophage induction to allow virion production.
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Evidence
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Anti-TerI2 (encoded by LMRG_02984) was independently identified as a second self-immunity protein opposing TerI activity. Its anti-defense function was initially obscured due to potential antisense transcriptional silencing of terI; however, co-expression experiments with a codon-modified synthetic terI (to eliminate antisense effects) confirmed that LMRG_02984 could restore virion production in TerI-expressing cells. Like Anti-TerI1, deletion of Anti-TerI2 (ECO_0001038) led to severely reduced virion production and DNA-less capsids, dependent on active terI. Bacterial adenylate cyclasebased two-hybrid assays demonstrated direct interaction between Anti-TerI2 and TerI, supporting a physical neutralization mechanism. Furthermore, overexpression of Anti-TerI2 restored phage infectivity during exogenous infection in TerI-expressing bacteria, underscoring its role as a functional anti-defense factor.
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Prophage ϕ10403S TerI defence system (active in Listeria monocytogenes)
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Relevant publication(s)
DOI
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10.1101/2025.02.27.640495
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Other components of the anti-defence system
Multicomponent System
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anti_rerI1
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Prophage ϕ10403S
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Defence system(s) inhibited by the protein
Defences
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TerI
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