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Mode of Action (MoA)
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directly binds to the Lon protease to prevent the degradation of the phage-encoded HNH endonuclease.
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Evidence
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Dap2 was identified as an anti-defense factor through infection assays using a Δdap2 mutant of phage PaoP5. Deletion of dap2 (ECO_0001038) reduced phage burst size and resulted in smaller plaques in Pseudomonas aeruginosa PAO1, independent of the T3SS system, indicating an additional anti-defense function. Pull-down assays (ECO_0006249) and mass spectrometry identified Lon protease as a Dap2 binding partner (ECO_0001096).
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MoA Category
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binds and inhibits host defence system
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Pseudomonas aeruginosa Lon-mediated antiviral defence
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Relevant publication(s)
DOI
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10.1101/2025.03.13.642734
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Other components of the anti-defence system
Multicomponent System
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dap1
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Pseudomonas phage PaoP5
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Defence system(s) inhibited by the protein
Defences
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Lon-mediated antiviral defence
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