Bacterial virus anti-defence systems · sequence & structure resource

Encyclopaedia of Bacterial Virus Anti-Defence Systems


Protein: NARP1_Namat (nicotinamide ADPR transferase)

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Overview

Mode of Action (MoA) synthesises NAD+ from ADPR-PP and nicotinamide
Evidence Expression of Namat homolog from phage SpβL1 catalyzed the formation of NAD⁺ from ADPR-PP and nicotinamide in vitro. LC–MS and MS/MS confirmed NAD⁺ as the product (ECO_0001096). Mutation of the predicted active site residue (R306G) abolished activity, indicating its essential catalytic role (ECO_0000015).
MoA Category synthesises and restores essential molecules depleted by bacterial defence
Subtype(s) of the defence system(s) inhibited by the protein Defence Subtype Bacillus subtilis type I Thoeris, DSR1, DSR2 and SEFIR. Escherichia coli SIR2–HerA
Relevant publication(s) DOI 10.1038/s41586-024-07986-w
Other components of the anti-defence system Multicomponent System adps
Known structure in PDB PDB ID -
Genome(s) encoding the protein Protein Source Escherichia phage JohannRWettstein (Bas63)
Defence system(s) inhibited by the protein Defences Thoeris, DSR, SIR2-HerA, SEFIR

3D structure

AlphaFold 3 model.

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Feature viewer - predicted secondary structure

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Pfam annotations

Pfam Name Pfam Acc Pfam Length Colour HMM Start HMM End Ali Start Ali End Env Start Env End Evalue
NAPRTase PF04095 239 1 132 190 352 190 382 5.7e-37
NAPRTase PF04095 239 124 217 429 531 415 548 5.7e-37

Sequence Viewer

Amino acid position: -

MTKSLYAVPAGLNADAYKSGHVYQYPSATEYLMFNLTPRSDKWFNSPLAIDGVVAFGIQRFVKDYLIDHWNATFFERDKKEAIDEILEVMNGVLGKDAIGREHWEALHDLGYLPVEVYAVEEGTVVPMRVPMIVFQNTVSGFHWVAGYLEDAFSAEIWKACTIATIALHYKRICKKWADLTCDNDLHLPYQCHDFAMRGMSGFTDDAFNAVGHLTSFKGTDSFPAVYTAKRIYGQSYPISDIGSSVPATEHSVMCANIAWEGGNELIEEERRFKGELQTFRRFLTETYPTGIASIVSDTYNFWRTVSEILPALRKEIMERDGKLVIRPDSGDPVHIVTGYKAIHLECAKKAYYEHLSKLEASDTMLDAVLNMKLENISYGIAGWLLSEGYEMVVDREDFEVADTVMLKNAYMVGSANVVTRPVAEIDGAIKTLYNIFGGTTNSKGFKVLDEHIGLIYGDSITLERANEILKRLYEMGFASSNVVFGVGSYTYQYMTRDTFAFAVKATLASIGGKEIMLAKDPKTDSGVKKSAFGGVSPMWDGDKLKAVDGYGFQSFADVLDHPACALRLVFSDSEQFGYTTLGDIRNNIDKQL