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Mode of Action (MoA)
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involved in repairing double-strand DNA breaks via recombination between short sequence repeats (single-strand-annealing recombinase).
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Evidence
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Deletion of any of these genes (vcrx091, vcrx092, and vcrx093) caused a 6–200 fold drop in conjugation efficiency in the presence of CRISPR targeting (ECO_0007379). Evasion was RecA-independent, consistent with single-strand annealing-type repair. The effect was rescued by complementation in the recipient only, showing the repair happens post-entry. Repaired plasmids showed specific deletions between short direct repeats, confirming Bet/Exo-mediated recombination.
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MoA Category
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synthesises and restores essential molecules depleted by bacterial defence
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Vibrio cholerae type I CRISPR-Cas
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Relevant publication(s)
DOI
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10.1093/nar/gkaa518
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Other components of the anti-defence system
Multicomponent System
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vcrx091;
vcrx093
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Plasmid pVCR94
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Defence system(s) inhibited by the protein
Defences
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CRISPR-Cas
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