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Mode of Action (MoA)
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converts dihydroneopterin triphosphate (H2NTP) into 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) (the second step of synthesis of 7-cyano-7-deazaguanine, a precursor in the pathway that leads to various DNA modifications found in phages).
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Evidence
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Viral genes (e.g., folE, queD, queE from phage 9g) were expressed in corresponding E. coli deletion mutants (ΔfolE, ΔqueD, ΔqueE), restoration of queuosine (Q) modification in tRNA confirmed that these viral enzymes are functional analogs of their bacterial counterparts (ECO_0000017). Phages with modifications (e.g., dG+, dPreQ0, dPreQ1) showed varied resistance to various restriction enzymes.
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MoA Category
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modifies phage molecules to avoid recognition
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Type II RM (in vitro)
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Relevant publication(s)
DOI
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10.1038/s41467-019-13384-y
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Other components of the anti-defence system
Multicomponent System
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quec;
fole;
quee
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Known structure in PDB
PDB ID
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-
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Genome(s) encoding the protein
Protein Source
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Enterobacteria phage 9g
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Defence system(s) inhibited by the protein
Defences
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RM
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