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Mode of Action (MoA)
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converts 2′-deoxycytidylate (or 2′-deoxycytidine-5′-monophosphate, dCMP) into 5-hydroxymethyl-dCMP (a step in DNA hypermodification).
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Evidence
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Crystal structure of T4 deoxycytidylate hydroxymethylase with dCMP shows the direct binding (ECO_0001034). Substitution of Asp179 to Asn drastically reduced activity on dCMP (~15,000-fold decrease in k_cat/K_M), but increased activity on dUMP, suggesting Asp179 is crucial for dCMP specificity and catalysis.
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MoA Category
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modifies phage molecules to avoid recognition
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Subtype(s) of the defence system(s) inhibited by the protein
Defence Subtype
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Escherichia coli RM
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Relevant publication(s)
DOI
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10.1093/emboj/18.5.1104
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Other components of the anti-defence system
Multicomponent System
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dnmp;
bgt
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Known structure in PDB
PDB ID
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1B5E_A
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Genome(s) encoding the protein
Protein Source
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Enterobacteria phage T4
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Defence system(s) inhibited by the protein
Defences
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RM
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View homologs from eukaryotic dsDNA viruses
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